Life Sciences Semiconductor Digital Illumination

Life Sciences

With the MicroPoint laser illumination and ablation systems and the Mosaic digital illumination systems, researchers are realizing unprecedented discoveries in the life sciences. Below are Technical Notes and Publications demonstrating a selection of these applications.

If a MicroPoint or Mosaic system was a critical tool in your research, Photonic Instruments would like to know. Please contact Photonic Instruments to share the results and method with others in the community on this website.

Technical Notes

The Technical Notes below describe how MicroPoint and Mosaic can be used in fluorescence microscopy, live cell imaging and laser ablation.

Laser Ablation in C. Elegans
FRAP
Haemeostasis
Haemeostasis – platelet thrombosis
Heat Shock Gene Promoter in Zebra Fish
In Vitro Fertilization
Release of Caged FITC

Publications

The journal articles below demonstrate how MicroPoint and Mosaic were used in ground breaking life sciences research

Title	Publication	System
An Essential Role for DYF-11/MIP-T3 in Assembling Functional Intraflagellar Transport Complexes	PLoS Genet 4(3): e1000044. doi:10.1371/ journal.pgen. 1000044	FRAP module is Mosaic Digital Illumination Systems with a 488nm 300mW laser; Volocity image acquisition software
APOBEC3G Multimers Are Recruited to the Plasma Membrane for Packaging into Human Immunodeficiency Virus Type 1 Virus-Like Particles in an RNA-Dependent Process Requiring the NC Basic Linker	JOURNAL OF VIROLOGY, May 2007, p. 5000–5013 Vol. 81, No. 10 0022-538X/07/$08.00 0 doi:10.1128/ JVI.02237-06 American Society for Microbiology	Mosaic Digital Illumination Systems with 488-nm laser, Nikon TE2000 microscope with 63x objective, Molecular Devices MetaMorph software
Dendrites of Distinct Classes of Drosophila Sensory Neurons Show Different Capacities for Homotypic Repulsion	Current Biology, Vol. 13, 618–626, OI 10.1016/S0960 -9822(03)00207-0	MicroPoint with tunable 337nm ablation laser source
In vivo dynamics of RNA polymerase II transcription	Nature Structural & Molecular Biology, VOLUME 14 NUMBER 9	3-D live cell imaging with Mosaic Digital Illumination Systems and wide field microscope; Milliseconds after bleaching, 3-D stacks were acquired every 3 s for 120 s
Intraflagellar Transport Protein 27 Is a Small G Protein Involved in Cell-Cycle Control	Current Biology 17, 193–202, DOI 10.1016/ j.cub.2006.12.040	Mosaic Digital Illumination Systems with Argon laser, Nikon TE2000 microscope with 100x, 1.4 NA objective, images collected at 100 ms per frame
Mobility of Human Immunodeficiency Virus Type 1 Pr55Gag in Living Cells	JOURNAL OF VIROLOGY, Sept. 2006, p. 8796–8806 Vol. 80, No. 17 0022-538X/06/$08.00 0 doi:10.1128/ JVI.02159-05 American Society for Microbiology	Photoactivation with Mosaic Digital Illumination Systems,Olympus IX-70 inverted microscope, Intelligent Imaging Innovation Slidebook software
Regulation of Myosin II Dynamics by Phosphorylation and Dephosphorylation of Its Light Chain in Epithelial Cells	Molecular Biology of the Cell Vol. 18, 605–616, February 2007	MicroPoint laser ablation, Olympus IX81 microscope with UplanApo 40x/1.00 OilIris Ph3 lens
TRIMalpha Cytoplasmic Bodies Are Highly Dynamic Structures	Molecular Biology of the Cell Vol. 18, 2102–2111, June 2007	Mosaic Digital Illumination Systems with 474nm light source

APPLICATIONS

Fluorescence Recovery After Photobleaching (FRAP)
Fluorescence Energy Resonant Transfer (FRET)
Photoactivation
Photobleaching
Photoswitching
Photoconverting
Total Internal Reflection Fluorescence (TIRF)
Cell Regeneration/Degeneration
Release of Caged Compounds
Drug Delivery
Thrombosis
Free Radical Release
Chromophore Assisted Laser Inactivation (CALI)